Sftpc creert2

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August undefined, 2024

Web南模生物. Language: 中文 . English; 日本語; 한국어; 账户设置; 退出 Web12 Jul 2024 · SFTPC creERT2 mice were generated as previously described . To generate the Gli2 creERT2-tdT mouse, a CreERT2-2A-tdTomato expression cassette was inserted …

Identifying the variables that drive tamoxifen‐independent CreERT2 …

Web25 Sep 2012 · A number of tissue-specific Cre-ER T2 mouse models have been reported [7], [8], [9]. In this study, we developed a novel SPC-Cre-ER T2 mouse model which can be … Web4 Nov 2024 · SftpcCreERT2/+;tdTomatoflox/flox mice were used for the labelling of AT2 cells and labelled subpopulations were analysed by flow cytometry, quantitative PCR, assay for transposase-accessible chromatin using sequencing (ATAC-seq), gene arrays, pneumonectomy and culture of precision-cut lung slices. law and order fear and loathing

Early lineage specification defines alveolar epithelial ontogeny in the murine lung …

WebBecause SFTPC mutations are rare (<5%) in IPF ( 14 ), a more generalized role for ER stress in AEC dysfunction and subsequent fibrosis remains to be established. The chaperone … Web10 Jan 2024 · Lungs from WT, Sftpc CreERT2 R26R EYFP, and Axin2 CreERT2–TdTomato mice were processed into single-cell suspensions as indicated above. WT AT2 cells: AT2 … WebSPC-Cre-ERT2 transgenic mouse for temporal gene deletion in alveolar epithelial cells Although several Cre-loxP-based gene knockout mouse models have been generated for … law and order female da cast

Longitudinal single cell transcriptomics of lung regeneration reveals …

WebSftpc Name surfactant associated protein C Synonyms Bricd6, pro-SpC, Sftp2, Sftp-2, SPC, SP-C Feature Type protein coding gene IDs MGI:109517 NCBI Gene: 20389 Alliance gene … Web29 Jul 2024 · SftpcCreERT2+/− mice were used as controls. b Genotyping of DNA extracted from CD45 (−)EpCAM (+) cells and CD45 (-)EpCAM (−) cells isolated from control and … law and order fbi castWebSftpc CreERT2/+;Tomato flox/+ (control), Sftpc CreERT2/+;Tomato flox/+;Fgfr2b flox/flox or Sftpc CreERT2/+; Tomato flox/+; Fgf10 +/-(experimental) mice are fed with tamoxifen food for one week followed by a chase period with normal food. Sham or left lung pneumonectomy are carried out in control and experimental mice ... ka-bar bridge breacher tool

"WebUsing cell state trajectory modeling, guided by RNA velocity of single cells from 18 timepoints and lineage tracing Sox2-CreERT2 and Sftpc-CreERT2 mice, we show that alveolar and airway stem cells converge into Krt8+ ADI cells and delineate gene expression dynamics during this process. " - Sftpc creert2

Sftpc creert2

Web将IRES-CreERT2敲入到小鼠Sftpc基因3'UTR中，他莫昔芬诱导型Cre重组酶CreERT2受内源Sftpc基因启动子驱动。诱导后，在II型肺泡细胞中表达Cre活性。应用领域：II型肺泡细胞Cre工具鼠 Web5 May 2024 · To explore this possibility we lineage-labeled AT2 cells and BASCs using an Sftpc CreERT2;mTmG mouse line. We found that labeling the BASCs required high doses …

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Web19 Feb 2024 · To assess this, we determined the relative contribution of AT1 and AT2 progenitor cells to the mature AT1 and AT2 lineages by using established AT1- and AT2-specific promoter-driven inducible Cre recombinases, HopxCreERTand SftpcCreERT2(23–27). Web19 May 2024 · a Tamoxifen treatment timeline of Sftpc CreERT2/+; Fgfr2b +/+; tdTomato flox/flox and Sftpc CreERT2/+; Fgfr2b flox/flox; tdTomato flox/flox mice. b Flow cytometry analysis of the percentage of tdTomato Pos cells in Ctrl. and Exp. on day 7 and day 14. Note the expansion of the E-IAAPs as well as the decrease in tdTom+/Epcam+ in Exp. lungs.

Web12 Jul 2024 · Immunofluorescence staining of the lung showed that the HGF receptor Met was expressed almost exclusively in the SFTPC + distal alveolar stem cells that were adjacent to Gli2 + distal mesenchyme in vivo ( Figure 4F ), suggesting that Hh activation in the distal alveolar mesenchyme might regulate HGF activation of MET in the alveolar … Web6 Dec 2024 · Tg (SFTPC-cre/ERT2)F42Kfx Transgene Detail MGI Mouse (MGI:5465199) Search All Search Tools Genes Genes & Markers Query Batch Query JBrowse Genome …

Web3 Mar 2024 · We lineage-labeled AT2 cells using SftpcCreERT2; R26REYFP mice and subjected them to acute lung injury ( Figure 4 D). Ten days after the injury, the lineage-labeled AT2 cells expressed higher levels of AT1-specific integrins ( Itgb5 and Itgb6 ), but not the AT2-specific integrin Itga6, than uninjured control cells ( Figure 4 E). Web28 May 2024 · The total AT2 population (Sftpc Pos Tom Pos cells) was isolated from lungs of 6-8 weeks old Sftpc CreERT2/+; tdTomato flox/flox mice. For the single-cell …

WebWhen challenged with s.c. bleomycin, tamoxifen- treated Etv5 fl/-Sftpc CreERT2/+ Rosa tdTomato/+ mice tended to de- velop more extensive and severe lung lesions than control mice (Fig. 4 A-D and ...

Web19 May 2024 · a Tamoxifen treatment timeline of Sftpc CreERT2/+; Fgfr2b +/+; tdTomato flox/flox and Sftpc CreERT2/+; Fgfr2b flox/flox; tdTomato flox/flox mice. b Flow cytometry … ka-bar becker companionWeb19 Oct 2024 · The Sftpc-CreERT2 mouse line has been used previously to track AT2 cell fate (65, 66). Gramd2-CreERT2 mice were generated by homologous recombination insertion of a cassette including CreERT2- downstream of … kabar becker bk2 accessoriesWeb10 Jun 2013 · For lineage-tracing AEC2s, we have used our Sftpc-CreER T2 allele in which a cassette encoding tamoxifen-activated (Tmx-activated) CreER is inserted into the … law and order feb 23http://www.labmodel.cn/mod_product-view-p_id-4491.html kabar clearanceWeb20 Jan 2024 · Quantitatively, AT2 cells from Sftpc CreERT2; Rosa26 miR-302 lungs exhibited increased mitochondrial area and decreased cristae density per mitochondrion, compared … kabar cryptocurrency hari iniWeb1 Feb 2024 · SFTPC-CreERT2;R26ReYFP at 7 days of culture. Membrane in magenta, SFTPC in green and nuclei in grey. Why I like this preprint. 3D cultures of AT2 cells are currently being used more and more for disease modeling of lung diseases involving the alveoli. From lung adenocarcinoma to COVID, there are multiple deadly diseases that use AT2 ... ka bar becker bk9 sheathWebOur results with the Sftpc CreERT2/+ ;Tomato flox/+ mice (PNX vs. SHAM) suggest that Fgf signaling is activated in AECII cells during PNX. Our ongoing experiments allow us to … ka-bar cane review