Phospho-axl
WebImmunoprecipitation of Phospho-Axl (Tyr698) protein from NCI-H1299 cells treated with GAS6 (400 ng/ml) and H 2 O 2 (4 mM) for 10 min. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900, lane 3 is Phospho-Axl (Tyr698)/Mer (Tyr749)/Tyro3 (Tyr681) Rabbit mAb, and lane 4 is Axl (C89E7) Rabbit mAb #8661. … WebLigand binding at the cell surface induces dimerization and autophosphorylation of AXL. Following activation by ligand, AXL binds and induces tyrosine phosphorylation of PI3-kinase subunits PIK3R1, PIK3R2 and PIK3R3; but also GRB2, PLCG1, LCK and PTPN11. Other downstream substrate candidates for AXL are CBL, NCK2, SOCS1 and TNS2.
Phospho-axl
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WebMay 31, 2024 · C, Western blot analysis of total and phospho-AXL in GSC267 cells treated with 2 or 8 ng/mL of recombinant PROS1. D, Western blot analysis of total- and pAXL in GSC267 cells starved for 24 hours, followed by incubation with conditioned medium treated with 1 or 10 μg/mL of PROS1 blocking peptide. E, Immunoprecipitation (IP) analysis of … WebIn enzymology, an acyl-phosphate-hexose phosphotransferase (EC 2.7.1.61) is an enzyme that catalyzes the chemical reaction. acyl phosphate + D-hexose an acid + D-hexose …
WebThe Human Phospho-Axl DuoSet IC ELISA detects ligand-induced Axl tyrosine phosphorylation. A172 cells were untreated or treated with Gas-6-containing conditioned media1 from the human lung cell line, WI38, for 15 minutes. ELISA and IP-Western blot (inset) analyses were done using 100 µg and 400 µg of lysate, respectively.
WebPhospho-Axl (Tyr698)/Mer (Tyr749)/Tyro3 (Tyr681) (D6M4W) Rabbit mAb detects immunoprecipitated or transfected levels of Axl/Mer/Tyro3 protein phosphorylated at Axl … WebJul 17, 2024 · This comparison was performed on AXL and/or EGFR phospho-modulated proteins showing a normalized Log2 (Avg (H/L ratio)) ≤ −0.5 or ≥0.5 at either time points.
WebAn immobilized capture antibody specific for Axl binds both phosphorylated and unphosphorylated Axl. After washing away unbound material, an HRP-conjugated detection antibody is used to detect only phosphorylated receptor, utilizing a standard HRP format. Complete Your Research Human Total Axl DuoSet IC ELISA Cat # DYC1643-2 Citations (2)
WebNov 1, 2024 · Antibodies for Western blots were as follows: antibodies to AXL (C89E7), phospho-p44/42 MAPK (Erk1/2; Thr202/Tyr204) D13.14.4E, p44/42 MAPK (Erk1/2; L34F12), phospho-Akt (Ser473; D9E), Akt (pan; 40D4) were from Cell Signaling Technology. Phospho-AXL-y779-antibody was from R&D Systems. grabo teatarPhospho-Axl (Tyr702) (D12B2) Rabbit mAb detects endogenous levels of Axl only when phosphorylated at Tyr702. This antibody may also cross-react with other overexpressed, related tyrosine-phosphorylated tyrosine kinases, such as EGFR. Species Reactivity: Human Species predicted to react based on 100% sequence homology: grab options chainWebJan 7, 2024 · The Human Phospho-Axl (Y779) Antibody from R&D Systems is a rabbit polyclonal antibody to Axl. This antibody reacts with human. The Human Phospho-Axl (Y779) Antibody has been validated for the following applications: Western Blot, Immunohistochemistry, Immunocytochemistry. target : Axl. chilis lake pleasant parkwayWebJun 13, 2024 · Receptor tyrosine kinase AXL (RTK-AXL) is regarded as a suitable target in glioblastoma (GBM) therapy. Since AXL kinase inhibitors are about to get approval for … grabo seam setterWebFeb 7, 2010 · The antibody pair provided in this kit recognizes Human AXL phosphorylated at site Tyrosine-779. Storage Stability: Rapidly measure phosphorylated protein in lysates; … chilislearningWebImmunofluorescent analysis of Phospho-P38 pThr180/pTyr182 Antibody was done on 70% confluent log phase SHSY5Y cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. graboplyWebPhospho-AXL (Tyr698, Tyr702, Tyr703) Rabbit anti-Human Mouse Rat, Polyclonal, Bioss Rabbit Polyclonal Antibody Supplier: Bioss BS5181R Catalog No. 50-198-6392 $443.25 / Each of 1 Qty Check Availability Add to cart Description Specifications Description Axl is a member of the TAM receptor family, which also includes also MerTK and TYRO3. grab or uber in perth